Buccal Swab Collection for Genotyping Assay

SPECIMEN REQUIREMENTS
  • An ideal choice of swabs is a sterile DNA-free polyester flock swab in a transport tube
  • Use of cotton swabs may result in lower DNA yields or DNA that contains PCR inhibitors
  • Specimen must be properly labeled prior to collection with at least two unique identifiers, such as:
    • Patient’s first and last name
    • Patient’s identification number
    • Sample identification number
  • Based on current stability data, sample stability has been established for 60 days at ambient temperature (18°C -25 °C)
  • Sample paper work, Testing Agreement (TA), should be completed with the following information:
    • First name and last name of patient
    • Patient and/or physician signature
    • Unique identifiers (patient’s identification number and sample identification number)
    • Patient’s date of birth (DOB)
    • Patient’s gender
    • Physician information, including account address and National Provider Identifier (NPI)
    • Date of service
    • Test Selection
BUCCAL SWAB COLLECTION PROCEDURE

Wear appropriate protective clothing and gloves when handling swabs

  1. Carefully remove the two swabs from its tube.
  2. Collect one swab at a time, and hold the swab as close to its head as is comfortable for the patient
  3. Ask the patient to open his/her mouth wide.
  4. Vigorously rub the inside of each cheek for 30 seconds, this will collect loose cells for DNA extraction
  5. When swabbing the patient, avoid the gums, teeth, and tongue
  6. Place both swabs back in its original tube, avoid touching the swab tip with gloves or against any surface
  7. Place tube and accompanying paperwork into individual sample bag
  8. Place specimen bag into Fed Ex Clinical Pak
  9. Store at room temperature (18°-25° C) until Fed Ex pick up
CRITERIA FOR SAMPLE REJECTION
  • Missing identifiers
  • Mixed identifiers and/or mixed paperwork
  • Sample stability expired
  • Sample with mold growth
  • Broken sample container exposing the specimen to the environment
  • Sample duplicates
  • Testing Agreement (TA) is unreadable (too many mistakes/improper corrections)
  • Patients who are undergoing a medical treatment, such as chemotherapy, which will reduce white blood cells
  • Patients who have received a bone marrow transplant; it would be unclear whether the results were based on DNA from the patient or the donor